Mouse Serine/threonine-protein kinase mTOR (mTOR) ELISA Kit

SKU: orb782438

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MTOR(Serine/threonine-protein kinase mTOR).

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MTOR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MTOR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MTOR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MTOR in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.127 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

FRAP ELISA Kit, FRAP1 ELISA Kit, FRAP2 ELISA Kit, MTOR ELISA Kit, RAFT1 ELISA Kit, RAPT1 ELISA Kit, FK506 Binding Protein 12 Rapamycin Associated Protein ELISA Kit, Mammalian Target Of Rapamycin ELISA Kit, FKBP12-Rapamycin Complex-Associated Protein 1 ELISA Kit, Rapamycin and FKBP12 target ELISA Kit

Quick Database Links

UniProt

Q9JLN9