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Catalog Number | orb429472 |
---|---|
Category | Proteins |
Description | Recombinant of mouse SDF1 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Stromal Cell-Derived Factor-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu |
Source | Escherichia Coli |
Biological Activity | The specific activity as determined by its ability to chemoattract human monocytes at 50-100ng/ml corresponding to a Specific Activity of 10,000-20,000IU/mg. |
Storage | Stability: Lyophilized SDF-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Buffer/Preservatives | The CXCL12 protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives. |
Alternative names | SDF-1, CXCL12, Pre-B cell growth-stimulating facto Read more... |
Note | For research use only |
Application notes | Chemokines |
Expiration Date | 6 months from date of receipt. |
> 97% as determined by SDS-PAGE and HPLC. | |
8.0 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
8.5 kDa | |
E.Coli |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |