Mouse S1PR2(Sphingosine 1 Phosphate Receptor 2) Microsample ELISA Kit

SKU: orb2997605

Description

Mouse S1PR2(Sphingosine 1 Phosphate Receptor 2) Microsample ELISA Kit

Research Area

Signal Transduction

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S1PR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S1PR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S1PR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S1PR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Mouse
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.111 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

EDG5; AGR16; EDG-5; Gpcr13; H218; LPB2; S1P2; Endothelial Differentiation,Sphingolipid G-Protein-Coupled Receptor 5; Sphingosine 1-phosphate receptor Edg-5

Quick Database Links

UniProt

P52592