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Mouse S100 Calcium Binding Protein (S100) ELISA Kit

Mouse S100 Calcium Binding Protein (S100) ELISA Kit

Catalog Number: orb2667810

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2667810
CategoryAssays and Kits
DescriptionMouse S100 Calcium Binding Protein (S100) ELISA Kit
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S100. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S100. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S100 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeSandwich
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.058 ng/mL
Sample TypesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Alternative namesS100-A1; S100A1; S100-Alpha; S-100 Protein; S-100
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NoteFor research use only
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