Mouse Regenerating Islet Derived Protein 4 (REG4) ELISA Kit

SKU: orb776243

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 4(REG4).

Research Area

Gastroenterology & Hepatology, Infectious Disease & Virology, Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 3000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse REG4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse REG4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse REG4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse REG4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 46.88-3000 pg/mL
• Sensitivity: 17.53 pg/mL
• Concentration: 3000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GISP ELISA Kit, REG-IV ELISA Kit, RELP ELISA Kit, Regenerating Gene Type IV ELISA Kit, Gastrointestinal Secretory Protein ELISA Kit, REG-like protein ELISA Kit

Quick Database Links

UniProt

Q9D8G5