Mouse Receptor Associated Protein of the Synapse (RAPSN) ELISA Kit

SKU: orb781079

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Associated Protein Of The Synapse(RAPSN).

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse RAPSN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RAPSN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse RAPSN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RAPSN in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.54 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CMS1D ELISA Kit, CMS1E ELISA Kit, RNF205 ELISA Kit, Rapsyn ELISA Kit, Acetylcholine receptor-associated 43 kDa protein ELISA Kit, 43 kDa postsynaptic protein ELISA Kit, RING finger protein 205 ELISA Kit

Quick Database Links

UniProt

P12672