Mouse Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) ELISA Kit

• Catalog Number: orb780494
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, DNA Activated, Catalytic Polypeptide(PRKDC).
• Reactivity: Mouse
• Concentration: 20 ng/mL
• UniProt ID: P97313
• Tested applications: ELISA
• Application notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PRKDC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRKDC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PRKDC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PRKDC in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.107 ng/mL
• Sample Types: Tissue homogenates and other biological fluids.
• Alternative names: HYRC ELISA Kit, HYRC1 ELISA Kit, DNPK1 ELISA Kit,
• Note: For research use only