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Mouse Programmed Cell Death Protein 1 Ligand 1 (PDL1) ELISA Kit

Catalog Number: orb777621

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb777621
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Programmed Cell Death Protein 1 Ligand 1(PDCD1LG1).
ReactivityMouse
Concentration10 ng/mL
UniProt IDQ9EP73
Tested applicationsELISA
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PDL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PDL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PDL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PDL1 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.055 ng/mL
Sample Typesserum, plasma, tissue homogenates and other biological fluids
Alternative namesCD274 ELISA Kit, PDL1 ELISA Kit, B7-H ELISA Kit, B
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NoteFor research use only
Mouse Programmed Cell Death Protein 1 Ligand 1 (PDL1) ELISA Kit