Mouse Procollagen Ⅰ N-Terminal Propeptide (PⅠNP) ELISA Kit

• Catalog Number: orb2667678
• Category: Assays and Kits
• Description: Mouse Procollagen Ⅰ N-Terminal Propeptide (PⅠNP) ELISA Kit
• Application notes: standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PⅠNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PⅠNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PⅠNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PⅠNP in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 1.56-100 ng/mL
• Sensitivity: 0.5662 ng/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: P1NP; N-Propeptide Of Type I Procollagen; Procolla
• Note: For research use only