Mouse Procollagen II C-Terminal Propeptide (PIICP) ELISA Kit

SKU: orb779378

Description

The Mouse Procollagen II C-Terminal Propeptide (PIICP) ELISA Kit is designed to quantify Procollagen II C-Terminal Propeptide (PIICP) in mouse serum, plasma, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 4.69-300 ng/mL and a sensitivity of 1.45 ng/mL.

Research Area

Infectious Disease & Virology, Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PIICP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIICP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIICP in the samples is then determined by comparing the OD of the samples to the standard curve.

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Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 4.69-300 ng/mL
• Sensitivity: 1.45 ng/mL
• Concentration: 300 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

P2CP ELISA Kit, C-Propeptide Of Type II Procollagen ELISA Kit, Procollagen II Carboxy Terminal Propeptide ELISA Kit

Quick Database Links

Gene Symbol

PIICP