Mouse PDE1C(Phosphodiesterase 1C, Calmodulin Dependent) Microsample ELISA Kit

SKU: orb2997679

Description

Mouse PDE1C(Phosphodiesterase 1C, Calmodulin Dependent) Microsample ELISA Kit

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PDE1C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PDE1C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PDE1C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PDE1C in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.054 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Hcam3; Cam-PDE 1C; Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C

Quick Database Links

UniProt

Q64338