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Mouse Olfactomedin 4 (OLFM4) ELISA Kit

Mouse Olfactomedin 4 (OLFM4) ELISA Kit

Catalog Number: orb2667817

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2667817
CategoryAssays and Kits
DescriptionMouse Olfactomedin 4 (OLFM4) ELISA Kit
Range3.13-200 ng/mL
Assay Time3.5h
UniProt IDQ3UZZ4
Sensitivity1.02 ng/mL
Alternative namesOLM4; hGC1; hOLfD; OlfD; GW112; GC1; G-CSF-stimula
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NoteFor research use only
Application notesstandard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse OLFM4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OLFM4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse OLFM4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OLFM4 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma, tissue homogenates and other biological fluids
Expiration DatePlease enquire.