Mouse Nuclear Factor Kappa B p65 (NFκB-p65) ELISA Kit

• Catalog Number: orb866940
• Category: Assays and Kits
• Description: Sandwich Mouse NFKB-p65 ELISA Kit
• Reactivity: Mouse
• Concentration: 2000 pg/mL
• UniProt ID: Q04207
• Tested applications: ELISA
• Application notes: standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 12.2 pg/mL
• Sample Types: serum, plasma, tissue homogenates
• Alternative names: NF kappa B p65delta3 ELISA Kit, NFKB3 ELISA Kit
• Note: For research use only