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Mouse Nt5e protein
Catalog Number: orb594864
| Catalog Number | orb594864 |
|---|---|
| Category | Proteins |
| Description | Recombinant Mouse 5'-nucleotidase(Nt5e),partial (Active) |
| Tag | C-terminal 6xHis-tagged |
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.2 μm filtered 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% Glycerol, pH 7.5 |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Protein Sequence | WELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLFLDAGDQYQGTIWFTVYKGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAHQISGLFLPSKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQVPVVQAYAFGKYLGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLDNYSTQELGRTIVYLDGSTQTCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDEKNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRVPIYEPLEMDKVYKVTLPSYLANGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVVYPAVEGRIKF |
| Protein Length | Partial |
| UniProt ID | Q61503 |
| MW | 58.8 kDa |
| Application notes | Partial |
| Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
| Source | Mammalian cell |
| Biological Origin | Mus musculus (Mouse) |
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse CD73-His at 2μg/ml can bind Anti-Human CD73 mAb, the ED50 of Anti-Human CD73 mAb is 3.70 ng/ml. |
| Expression Region | 29-550aa |
| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
| Alternative names | 5'-nucleotidase;Ecto-5'-nucleotidase;CD73;5'-NT |
| Note | For research use only |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
- Item 1 of 3
Recombinant mouse CD73 protein, C-His (HEK293) [orb1516481]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
100 μg 
Mouse NT5E protein [orb392210]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Human cells
500 μg, 10 μg, 50 μgMouse NT5E Protein [orb427419]
Greater than 90% as determined by SDS-PAGE.
Sf9, Baculovirus cells
1 μg, 5 μg, 50 μgMouse NT5E Protein, His Tag [orb2699330]
> 95%, determined by SDS-PAGE
This protein contains the mouse NT5E (NP_0359811) without the propeptide, corresponding to the amino acid (Met 1-Lys 549) was expressed with a C-terminal polyhistidine tag and expressed from HEK293 Cells.
50 μg, 10 μg
