Mouse NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) ELISA Kit

• Catalog Number: orb782216
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NLR Family, Pyrin Domain Containing Protein 3(NLRP3).
• Tested applications: ELISA
• Reactivity: Mouse
• Range: 0.16-10 ng/mL
• Assay Time: 3.5h
• Concentration: 10 ng/mL
• UniProt ID: Q8R4B8
• Sensitivity: 0.052 ng/mL
• Alternative names: AVP ELISA Kit, AGTAVPRL ELISA Kit, AII ELISA Kit,
• Note: For research use only
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NLRP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NLRP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NLRP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NLRP3 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Expiration Date: Please enquire.

References

Hala A Ahmed 1, Ahmed A Shaaban 2, Mirhan N Makled 3, Tarek M Ibrahim G protein-coupled estrogen receptor selective agonist, G1, improves the molecular and biochemical markers in a cisplatin mouse model of CKD Chem Biol Interact ., (2024)

PubMed: 38795875

Applications

ELISA

Reactivity

Mouse

Sara H Hazem 1, Karim M Saad 2, Mahmoud M Samaha Protective effects of BTK inhibition by acalabrutinib on cisplatin-induced renal and testicular injury in mice: Modulation of mTOR/AMPK, NLRP3/GSDMD-N, and apoptotic pathways Int Immunopharmacol ., (2025)

PubMed: 39938312

Applications

ELISA

Reactivity

Mouse

Fahad O Alenezi 1, Manar A Nader 2, Dalia H El-Kashef 2, Marwa E Abdelmageed Dapansutrile mitigates concanavalin A- induced autoimmune hepatitis: Involvement of NLRP3/IL-1β and JNK/ p38 MAPK pathways Biomed Pharmacother, (2025)

PubMed: 40164046

Applications

ELISA

Reactivity

Mouse