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Catalog Number | orb752979 |
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Category | Proteins |
Description | Vascular Endothelial Growth Factor Mouse Recombinant produced in yeast is a disulfide-linked homodimer consisting of 2x165 amino acid polypeptide chains, having a molecular mass of approximately 40.0kDa each.VEGF is purified by proprietary chromatographic techniques. |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR |
Source | Saccharomyces cerevisiae |
Biological Activity | The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is 1.0-5.0 ng/ml. |
Storage | Stability: Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vascular Endothelial Growth Factor should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles |
Buffer/Preservatives | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. |
Alternative names | Vascular endothelial growth factor A, VEGF-A, Vasc Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 6 months from date of receipt. |
Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | |
Saccharomyces cerevisiae |