Cart summary

You have no items in your shopping cart.

Mouse Mucin-5 Subtype B (MUC5B) ELISA Kit

Catalog Number: orb1948219

DispatchUsually dispatched within 5-10 working days
$ 280.00
Catalog Numberorb1948219
CategoryAssays and Kits
DescriptionMouse Mucin-5 Subtype B (MUC5B) ELISA Kit
TargetMUC5B
ReactivityMouse
UniProt IDE9Q5I3
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MUC5B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MUC5B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MUC5B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MUC5B. You can calculate the concentration of Mouse MUC5B in the samples by comparing the OD of the samples to the standard curve.
Assay TypeSandwich
Assay Time3.5H
Range0.32-20ng/mL
Sensitivity0.19ng/mL
Sample TypesSerum, Plasma, Tissue homogenate and Other biological samples
Alternative namesMUC5B, MG1, MUC-5B, MUC9
NoteFor research use only
Mouse Mucin-5 Subtype B (MUC5B) ELISA Kit