Mouse Mothers Against Decapentaplegic Homolog 2/3 (Smad2/3) ELISA Kit

SKU: orb1496428

Description

The Mouse Mothers Against Decapentaplegic Homolog 2/3 (Smad2/3) ELISA Kit is designed to quantify Mothers Against Decapentaplegic Homolog 2/3 (Smad2/3) in mouse serum, plasma, tissue homogenates. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.059 ng/mL.

Research Area

Cancer Biology

Images & Validation

• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Smad2/3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Smad2/3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Smad2/3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Smad2/3 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.059 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

Smad 2/3