Mouse MIP 3b Protein

• Catalog Number: orb429446
• Category: Proteins
• Description: Recombinant of mouse MIP3 beta protein
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
• Solubility (25°C): It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-3b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be, Gly-Ala-Asn-Asp-Ala
• Source: Escherichia Coli
• Biological Activity: The Activity is calculated by the ability to chemoattract Human mature dendritic cells using a concentration range of 8-80 ng/ml.
• Storage: Stability: Lyophilized MIP-3b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL19 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles
• Buffer/Preservatives: The CCL19 was lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
• Alternative names: Small inducible cytokine A19, CCL19, Macrophage in
• Note: For research use only
• Application notes: Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.91 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of MIP-3b as a Reference Standard
• Expiration Date: 6 months from date of receipt.