Mouse Milk Fat Globule EGF Factor 8 (MFGE8) ELISA Kit

SKU: orb775244

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Milk Fat Globule EGF Factor 8(MFGE8).

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MFGE8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MFGE8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MFGE8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MFGE8 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

HMFG ELISA Kit, MFGM ELISA Kit, SED1 ELISA Kit, BA46 ELISA Kit, EDIL1 ELISA Kit, OAcGD3S ELISA Kit, SPAG10 ELISA Kit, hP47 ELISA Kit, Medin ELISA Kit, Lactadherin ELISA Kit, Sperm Associated Antigen 10 ELISA Kit, Sperm Surface Protein hP47 ELISA Kit, Milk fat globule-EGF factor 8 ELISA Kit

Quick Database Links

UniProt

P21956