Mouse Midline 1 (MID1) ELISA Kit

SKU: orb780934

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Midline 1(MID1).

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MID1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MID1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MID1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MID1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.054 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

BBBG1 ELISA Kit, Midin ELISA Kit, FXY ELISA Kit, GBBB1 ELISA Kit, OGS1 ELISA Kit, OS ELISA Kit, OSX ELISA Kit, RNF59 ELISA Kit, TRIM18 ELISA Kit, XPRF ELISA Kit, ZNFXY ELISA Kit, Opitz/BBB Syndrome ELISA Kit, RING finger protein 59 ELISA Kit, Tripartite motif-containing protein 18 ELISA Kit

Quick Database Links

UniProt

O70583