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| Catalog Number | orb779165 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Meprin A Alpha(MEP1a) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Meprin A Alpha(MEP1a). |
| Reactivity | Mouse |
| Concentration | 4000 pg/mL |
| UniProt ID | P28825 |
| Tested applications | ELISA |
| Application notes | standard: 4000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse MEP1a protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MEP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MEP1a in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 2.5h |
| Range | 62.5-4000 pg/mL |
| Sensitivity | 19.1 pg/mL |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | PPHA ELISA Kit, PABA Peptide Hydrolase ELISA Kit, Read more... |
| Note | For research use only |
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