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Mouse Mast Cell Tryptase (MCT) ELISA Kit

Catalog Number: orb1496500

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1496500
CategoryAssays and Kits
DescriptionMouse MCT ELISA kit
ReactivityMouse
Range31.25-2000 pg/mL
Assay Time3.5h
Concentration2000 pg/mL
Sensitivity13.2 pg/mL
Alternative namesTPSAB1; TPS1; TPS2; TPSB1; Mcp7; Mcp-7; Mcpt7; MMC
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NoteFor research use only
Application notesstandard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MCT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MCT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MCT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MCT in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates
Expiration DatePlease enquire.
Mouse Mast Cell Tryptase (MCT) ELISA Kit