Mouse Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit

SKU: orb781635

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Gamma(MIP1g).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MIP1g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MIP1g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MIP1g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MIP1g in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 3.2 pg/mL
• Concentration: 500 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CCL9 ELISA Kit, CCL10 ELISA Kit, SCYB9 ELISA Kit, CCF18 ELISA Kit, MRP2 ELISA Kit, C-C motif chemokine 9 ELISA Kit, Macrophage inflammatory protein-related protein 2 ELISA Kit, Small-inducible cytokine A9 ELISA Kit