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Mouse Lysophosphatidylcholine Acyltransferase 4 (LPCAT4) ELISA Kit

Catalog Number: orb780577

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb780577
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysophosphatidylcholine Acyltransferase 4(LPCAT4).
Tested applicationsELISA
ReactivityMouse
Range0.63-40 ng/mL
Assay Time3.5h
Concentration40 ng/mL
UniProt IDQ6NVG1
Sensitivity0.215 ng/mL
Alternative namesAYTL3 ELISA Kit, AGPAT7 ELISA Kit, LPAAT ELISA Kit
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NoteFor research use only
Application notesstandard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LPCAT4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LPCAT4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LPCAT4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LPCAT4 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesTissue homogenates and other biological fluids.
Expiration DatePlease enquire.
Mouse Lysophosphatidylcholine Acyltransferase 4 (LPCAT4) ELISA Kit