Mouse Lipoprotein A (Lpa) ELISA Kit

• Catalog Number: orb2667284
• Category: Assays and Kits
• Description: Mouse Lipoprotein A (Lpa) ELISA Kit
• Application notes: standard: 20 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Lp-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Lp-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Lp-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Lp-a in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 0.312-20 µg/mL
• Sensitivity: 0.128 µg/mL
• Sample Types: Serum, plasma and other biological fluids.
• Alternative names: LP; AK38; APOA
• Note: For research use only