Recombinant Mouse LIF Protein

SKU: orb3002423

Description

Recombinant Mouse Leukemia Inhibitory Factor is produced by our E.coli expression system and the target gene encoding Ser24-Phe203 is expressed.

Key Properties

• Expression System: E.coli
• Biological Activity: Cell Based Assay: Measured by the dose dependent inhibition of M1 cells. The ED50 for this effect is 0.33ng/ml(Regularly tested). The activity of Recombinant Mouse LIF Protein(Cat#orb3002423) was compared to Brand M.Measured by the dose dependent inhibition of M1 cells.
• Tag: Tag free
• Molecular Weight: Predicted: 19.9 KDa. Observed: 18 KDa, reducing conditions
• Expression Region: Ser24-Phe203
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Leukemia inhibitory factor;Differentiation-stimulating factor;lif;D factor

Quick Database Links

UniProt

P09056