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Description
Research Area
Immunology & Inflammation
Images & Validation
−
Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | Cell Based Assay: Measured by the dose dependent inhibition of M1 cells. The ED50 for this effect is 0.33ng/ml(Regularly tested). The activity of Recombinant Mouse LIF Protein(Cat#orb3002423) was compared to Brand M.Measured by the dose dependent inhibition of M1 cells. |
| Tag | Tag free |
| Expression Region | Ser24-Phe203 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| MW | Predicted: 19.9 KDa. Observed: 18 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Leukemia inhibitory factor;Differentiation-stimulating factor;lif;D factor
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Quick Database Links
UniProt
UniProt Details
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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