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Mouse Lactoferrin (LTF) ELISA Kit

Mouse Lactoferrin (LTF) ELISA Kit

Catalog Number: orb2667995

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2667995
CategoryAssays and Kits
DescriptionMouse Lactoferrin (LTF) ELISA Kit
UniProt IDP08071
Application notesstandard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LTF in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range6.25-400 ng/mL
Sensitivity2.14 ng/mL
Sample Typesserum, plasma, breast milk and other biological fluids
Alternative namesHLF2; LF; GIG12; Lactotransferrin; Growth-inhibiti
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NoteFor research use only
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