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Catalog Number | orb2667699 |
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Category | Assays and Kits |
Description | Mouse Kallikrein 1 (KLK1) ELISA Kit |
UniProt ID | P15947 |
Application notes | standard: 20 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse KLK1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KLK1 in the samples is then determined by comparing the OD of the samples to the standard curve |
Assay Time | 2.5h |
Range | 0.32-20 ng/mL |
Sensitivity | 0.14 ng/mL |
Sample Types | Serum, plasma and other biological fluids. |
Alternative names | KLKR; Klk6; HK1; Kallikrein 1,Renal/Pancreas/Saliv Read more... |
Note | For research use only |