Mouse ITGa3(Integrin Alpha 3) Microsample ELISA Kit

SKU: orb2998436

Description

Mouse ITGa3(Integrin Alpha 3) Microsample ELISA Kit

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITGa3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITGa3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITGa3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITGa3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.12 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD49c; CD49-C; ITG-A3; GAP-B3; GAPB3; MSK18; VCA-2; VL3A; VLA3a; FRP-2; Galactoprotein B3; Alpha 3 Subunit Of VLA-3 Receptor; CD49 antigen-like family member C

Quick Database Links

UniProt

Q62470