You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb775130 |
---|---|
Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 2, Intestinal(FABP2). |
Tested applications | ELISA |
Reactivity | Mouse |
Range | 0.16-10 ng/mL |
Assay Time | 3.5h |
Concentration | 10 ng/mL |
UniProt ID | P55050 |
Sensitivity | 0.055 ng/mL |
Alternative names | FABPI ELISA Kit, IFABP ELISA Kit, I-FABP ELISA Kit Read more... |
Note | For research use only |
Application notes | standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IFABP/FABP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IFABP/FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IFABP/FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IFABP/FABP2 in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |
Mouse | |
0.16-10ng/mL | |
0.1ng/mL |