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Mouse Intestinal Fatty Acid Binding Protein (IFABP/FABP2) ELISA Kit

Catalog Number: orb775130

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb775130
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 2, Intestinal(FABP2).
ReactivityMouse
Concentration10 ng/mL
UniProt IDP55050
Tested applicationsELISA
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IFABP/FABP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IFABP/FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IFABP/FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IFABP/FABP2 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.055 ng/mL
Sample TypesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Alternative namesFABPI ELISA Kit, IFABP ELISA Kit, I-FABP ELISA Kit
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NoteFor research use only
Mouse Intestinal Fatty Acid Binding Protein (IFABP/FABP2) ELISA Kit