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Mouse Interleukin 19 (IL19) ELISA Kit

Catalog Number: orb776813

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb776813
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 19(IL19).
Tested applicationsELISA
ReactivityMouse
Range7.82-500 pg/mL
Assay Time3.5h
Concentration500 pg/mL
UniProt IDQ8CJ70
Sensitivity3.2 pg/mL
Alternative namesMDA1 ELISA Kit, ZMDA1, IL-10C, NG.1 ELISA Kit, Mel
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NoteFor research use only
Application notesstandard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL19. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL19. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL19, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL19 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.
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