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Mouse Interleukin 12 p40 (IL-12p40) ELISA Kit

Catalog Number: orb776371

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb776371
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12B(IL12B).
Tested applicationsELISA
ReactivityMouse
Range15.63-1000 pg/mL
Assay Time3.5h
Concentration1000 pg/mL
UniProt IDP43432
Sensitivity5.7 pg/mL
Alternative namesp40 ELISA Kit, IL12-B ELISA Kit, CLMF2 ELISA Kit,
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NoteFor research use only
Application notesstandard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.
Mouse Interleukin 12 p40 (IL-12p40) ELISA Kit

  • Mouse IL12 p40 ELISA Kit [orb315048]

    Mouse

    7.8 pg/ml - 500 pg/ml

    4 pg/ml

    96 T