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Catalog Number | orb776371 |
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Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12B(IL12B). |
Tested applications | ELISA |
Reactivity | Mouse |
Range | 15.63-1000 pg/mL |
Assay Time | 3.5h |
Concentration | 1000 pg/mL |
UniProt ID | P43432 |
Sensitivity | 5.7 pg/mL |
Alternative names | p40 ELISA Kit, IL12-B ELISA Kit, CLMF2 ELISA Kit, Read more... |
Note | For research use only |
Application notes | standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |