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Mouse Interleukin 12 p40 (IL-12p40) ELISA Kit

Catalog Number: orb776371

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb776371
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12B(IL12B).
ReactivityMouse
Concentration1000 pg/mL
UniProt IDP43432
Tested applicationsELISA
Application notesstandard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range15.63-1000 pg/mL
Sensitivity5.7 pg/mL
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Alternative namesp40 ELISA Kit, IL12-B ELISA Kit, CLMF2 ELISA Kit,
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NoteFor research use only
Mouse Interleukin 12 p40 (IL-12p40) ELISA Kit

  • Mouse IL12 p40 ELISA Kit [orb315048]

    Mouse

    7.8 pg/ml - 500 pg/ml

    4 pg/ml

    96 T