Mouse Interleukin 1 Family, Member 9 (IL1F9) ELISA Kit

SKU: orb781786

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Family, Member 9(IL1F9).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL1F9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL1F9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL1F9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL1F9 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 2.9 pg/mL
• Concentration: 500 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

IL-1F9 ELISA Kit, IL36g ELISA Kit, IL-1H1 ELISA Kit, IL-1RP2 ELISA Kit, IL1E ELISA Kit, IL1H1 ELISA Kit, IL1RP2 ELISA Kit, Interleukin 36, Gamma ELISA Kit, Interleukin-1 Homolog 1 ELISA Kit, Interleukin-1 epsilon ELISA Kit, Interleukin 1-Related Protein 2 ELISA Kit

Quick Database Links

UniProt

Q8R460