Mouse Interleukin 1 Beta (IL-1β) High Sensitivity ELISA Kit

SKU: orb1945919

Description

Mouse Interleukin 1 Beta (IL-1β) High Sensitivity ELISA Kit

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-1β. You can calculate the concentration of Mouse IL-1β in the samples by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Mouse
• Sample Types: Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL
• Assay Type: Sandwich
• Assay Time: 3.5H
• Range: 0.16-10pg/mL
• Sensitivity: 0.09 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IL1β, IL1B, IL1-BETA, IL1F2, catabolin, IL1b, IL-1B, IL-1b

Standard curve for orb1945919 ELISA kit.

Quick Database Links

UniProt

P10749