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Mouse Intercellular Adhesion Molecule 1 (ICAM-1/CD54) ELISA Kit

Catalog Number: orb1806781

DispatchUsually dispatched within 5-10 working days
$ 280.00
Catalog Numberorb1806781
CategoryAssays and Kits
DescriptionMouse Intercellular Adhesion Molecule 1 (ICAM-1/CD54) ELISA Kit
TargetICAM-1/CD54
ReactivityMouse
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ICAM-1/CD54. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substMousee solution is added to each well. Only those wells that contain Mouse ICAM-1/CD54, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substMousee reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentMouseion of Mouse ICAM-1/CD54. You can calculate the concentMouseion of Mouse ICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve.
Assay Time3.5H
Range1.37-1000 ng/mL
Sensitivity0.82 ng/mL
Sample TypesSerum, Plasma and Other biological fluids
Alternative namesAntigen identified by monoclonal BB2,BB 2,BB2,CD 5
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NoteFor research use only
Mouse Intercellular Adhesion Molecule 1 (ICAM-1/CD54) ELISA Kit