Mouse Integrin Beta 1 (ITGb1) ELISA Kit

SKU: orb779159

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 1(ITGb1).

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITGb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITGb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITGb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITGb1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.111 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD29 ELISA Kit, MSK12 ELISA Kit, ITG-B1 ELISA Kit, FNRB ELISA Kit, GPIIA ELISA Kit, MDF2 ELISA Kit, MSK12 ELISA Kit, VLAB ELISA Kit, Fibronectin Receptor Beta Polypeptide ELISA Kit, Antigen CD29 Includes MDF2 ELISA Kit, Glycoprotein IIa ELISA Kit, VLA-4 subunit beta ELISA Kit

Quick Database Links

UniProt

P09055