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Mouse Insulin Like Growth Factor Binding Protein 1 (IGFBP1) ELISA Kit

Catalog Number: orb776124

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb776124
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 1(IGFBP1).
ReactivityMouse
Concentration10 ng/mL
UniProt IDP47876
Tested applicationsELISA
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IGFBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IGFBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IGFBP1 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeSandwich
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.06 ng/mL
Sample TypesSerum, plasma and other biological fluids.
Alternative namesIBP1 ELISA Kit, AFBP ELISA Kit, IGF-BP25 ELISA Kit
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NoteFor research use only
Mouse Insulin Like Growth Factor Binding Protein 1 (IGFBP1) ELISA Kit

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