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Mouse Inhibitory Subunit of NF Kappa B Alpha (IkBa) ELISA Kit

Catalog Number: orb778204

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb778204
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibitory Subunit Of NF Kappa B Alpha(IkBa).
Tested applicationsELISA
ReactivityMouse
Range0.16-10 ng/mL
Assay Time3.5h
Concentration10 ng/mL
UniProt IDQ9Z1E3
Sensitivity0.057 ng/mL
Alternative namesNFKBIA ELISA Kit, IKB-A ELISA Kit, MAD-3 ELISA Kit
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NoteFor research use only
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IkBa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IkBa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IkBa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IkBa in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesTissue homogenates, cell lysates and other biological fluids.
Expiration DatePlease enquire.
Mouse Inhibitory Subunit of NF Kappa B Alpha (IkBa) ELISA Kit