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Catalog Number | orb594835 |
---|---|
Category | Proteins |
Description | Recombinant Mouse Interleukin-4(Il4),partial (Active) |
Tag | Tag-Free |
Form/Appearance | Lyophilized powder |
Purity | Greater than 95% as determined by SDS-PAGE. |
MW | 13.4 kDa |
UniProt ID | P07750 |
Protein Sequence | HGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCRASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS |
Protein Length | Partial |
Source | E.coli |
Expression System | 23-140aa |
Biological Activity | The ED50 as determined in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells is less than 0.01 ng/ml. |
Expression Region | 23-140aa |
Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, 5% Trehalose, pH 6.5. |
Alternative names | Interleukin-4;B-cell IgG differentiation factor;B- Read more... |
Note | For research use only |
Application notes | Partial |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
> 97% as determined by SDS-PAGE and HPLC. | |
13.5 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
14.6 kDa | |
Mammalian cell |
Unconjugated | |
95% | |
51 kDa | |
Mouse IL-4 R alpha, Fc Tag (orb334907) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Arg 233 (Accession # NP_001008700). |
Unconjugated | |
95% | |
26.3 kDa | |
Mouse IL-4 R alpha, His Tag (orb334908) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Arg 233 (Accession # NP_001008700). |
Unconjugated | |
90% | |
15.0 kDa | |
Human IL-4, premium grade (orb257598) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P05112-1). |
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