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Catalog Number | orb707221 |
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Category | Proteins |
Description | Recombinant Mouse IL-12 Protein is produced by mammalian expression system and the target gene encoding Met23-Ser335 & Arg23-Ala215 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Mouse |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | IL12A; IL12B |
Entrez | 16160, 16159 |
UniProt ID | P43431, P43432 |
Protein Sequence | MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS&RVIPVS GPARCLSQSR NLLKTTDDMV KTAREKLKHY SCTAEDIDHE DITRDQTSTL KTCLPLELHK NESCLATRET SSTTRGSCLP PQKTSLMMTL CLGSIYEDLK MYQTEFQAIN AALQNHNHQQ IILDKGMLVA IDELMQSLNH NGETLRQKPP VGEADPYRVK MKLCILLHAF STRVVTINRV MGYLSSA |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Alternative names | NKSF1 protein, Interleukin-12 subunit alpha protei Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
≥90% as determined by SDS-PAGE | |
This protein contains the mouse Il12a(Met1-Ala215) was fused with the C-terminal His Tag. This protein contains the mouse Il12b(Met1-Ser335) was fused with the C-terminal Twin-Strep Tag and expressed in Mammalian. |
≥90% as determined by SDS-PAGE | |
This protein contains the mouse Il12a(Met23-Ser335&Arg23-Ala215) was fused without Tag and expressed in Mammalian cells. |
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