Recombinant Mouse IL-7 Protein

SKU: orb3002426

Description

Recombinant Mouse Interleukin-7 is produced by our Mammalian expression system and the target gene encoding Glu26-Ile154 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Activity: BLI: Loaded Mouse IL-7RA-Fc(Cat#orb2994406) on Protein A Biosensor, can bind Mouse IL-7-His(Cat#orb3002426) with an affinity constant of 18.9 nM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL).The ED50 for this effect is 60-1000 pg/ml. (QC verified)
• Tag: C-6His
• Molecular Weight: Predicted: 15.9 KDa. Observed: 22-28 KDa, reducing conditions
• Expression Region: Glu26-Ile154
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
• Endotoxins: < 1 EU/µg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IL-7; IL-7 interleukin-7; interleukin-7; Lymphopoietin -1; PBGF

Quick Database Links

UniProt

P10168