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Catalog Number | orb1710804 |
---|---|
Category | Proteins |
Description | Mouse IL-21 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Pro 25 - Ser 146 (Accession # Q9ES17). |
Target | IL-21 |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Mouse |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
UniProt ID | Q9ES17 |
MW | 16.3 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.3 kDa. The protein migrates as 20 kDa and 23-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Mouse |
Biological Activity | Immobilized Mouse IL-21 Protein, His Tag (Cat. No. ) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-21 R Protein, His, (Cat. No. ILR-M82E3) with a linear range of 0.3-20 ng/mL (QC tested). |
Expression Region | Pro 25 - Ser 146 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
Immobilized Mouse IL-21 Protein, His Tag (Cat. No. orb1710804) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-21 R Protein, His, Avitag with a linear range of 0.3-20 ng/mL (QC tested).
> 90% as determined by SDS-PAGE | |
16.8 kDa |
Greater than 95.0% as determined by SDS-PAGE. | |
HEK293 Cells |
≥90% as determined by SDS-PAGE | |
This protein contains the mouse Il21(Pro25-Ser146) was fused with the N-terminal His Tag and expressed in E. coli. |
98.00% | |
17.9 kDa (predicted) same as Tris-Bis PAGE result. |