Mouse IL-21 Protein, His Tag

• Catalog Number: orb1710804
• Category: Proteins
• Description: Interleukin-21 (IL-21) is a secreted protein which belongs to the IL-15 / IL-21 family. Interleukin-21 / IL-21 belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL21R) and the common cytokine receptor gamma chain (gamma(C)). Interleukin-21 / IL-21 impacts a number of cell types, including CD8+ memory T cells, NK cells and subsets of CD4 memory T cells. The IL-21R is widely distributed on lympho-haematopoietic cells. IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
• Reactivity: Mouse
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 16.1 kDa
• Target: IL-21
• Source: Mouse IL-21 Protein, His Tag (orb1710804) is expressed from human 293 cells (HEK293). It contains AA Pro 25 - Ser 146 (Accession # Q9ES17).
• Expression System: HEK293
• Biological Activity: Immobilized Mouse IL-21 Protein, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-21 R Protein, His, with a linear range of 0.3-20 ng/mL (QC tested).
• Expression Region: Pro 25 - Ser 146
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.1 kDa. The protein migrates as 18 kDa and 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Mouse IL-21 Protein, His Tag (Cat. No. orb1710804) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-21 R Protein, His, Avitag with a linear range of 0.3-20 ng/mL (QC tested).