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Catalog Number | orb762436 |
---|---|
Category | Proteins |
Description | Mouse IL-2, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Gln 169 (Accession # P04351-1). |
Target | IL-2 |
Conjugation | Unconjugated |
Tag | N-10×His |
Reactivity | Mouse |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
Protein Sequence | NP_032392.1 |
UniProt ID | P04351 |
MW | 19.1 kDa |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 19.1 kDa. The protein migrates as 22-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Mouse |
Biological Activity | Mouse IL-2, His Tag (Cat. No. ) immobilized on CM5 Chip can bind Mouse IL-2 R beta, Fc Tag (Cat. No. ILB-M5253) with an affinity constant of 36.2 μM as determined in a SPR assay (Biacore 8K) (Routinely tested). |
Expression Region | Ala 21 - Gln 169 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_032392.1 |
Mouse IL-2, His Tag (Cat. No. orb762436) immobilized on CM5 Chip can bind Mouse IL-2 R beta, Fc Tag with an affinity constant of 36.2 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
> 95% by SDS-PAGE. | |
Recombinant Mouse IL-2RA/IL-2 R alpha/CD25 Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Glu22-Lys236) of mouse IL-2RA/IL-2 R alpha/CD25 (Accession #P01590) fused with a 6��His Tag at the C-terminus. |
98.00% | |
20.1 kDa (predicted). Due to glycosylation, the protein migrates to 27-30 kDa based on Tris-Bis PAGE result. |
98.00% | |
20.1 kDa (predicted). Due to glycosylation, the protein migrates to 27-30 kDa based on Tris-Bis PAGE result. |