Recombinant Mouse IL-2 Protein

SKU: orb3002463

Description

Recombinant Mouse Interleukin-2 is produced by our E.coli expression system and the target gene encoding Ala21-Gln169 is expressed.

Key Properties

• Expression System: E.coli
• Biological Activity: BLI: Loaded Human IL-2RA-Fc(Cat#orb2994463) on Protein A Biosensor, can bind Mouse IL-2(Cat#orb3002463) with an affinity constant of 0.17 uM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells.The specific activity of Recombinant Mouse IL-2 is ≥1×107 IU/mg. (QC verified)
• Tag: Tag free
• Molecular Weight: Predicted: 17.4 KDa. Observed: 17 KDa, reducing conditions
• Expression Region: Ala21-Gln169
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
• Endotoxins: < 0.01 EU/µg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM Sodium Citrate,0.02%Tween 80,4%Mannitol,pH 3.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

aldesleukin; interleukin 2; interleukin-2; IL-2; IL2; T-cell growth facter; T cell growth factor; TCGF

Quick Database Links

UniProt

P04351