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Mouse IL-10 Protein, His Tag
Catalog Number: orb334904
| Catalog Number | orb334904 |
|---|---|
| Category | Proteins |
| Description | Mouse IL-10, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Ser 178 (Accession # NP_034678). |
| Target | IL-10 |
| Conjugation | Unconjugated |
| Tag | N-6×His |
| Reactivity | Mouse |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 92% |
| Protein Sequence | NP_034678 |
| UniProt ID | P18893 |
| MW | 19.6 kDa |
| Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 19.6 kDa. The protein migrates as 22-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Mouse |
| Expression Region | Ser 19 - Ser 178 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_034678 |
SDS-PAGE analysis of Mouse IL-10 protein
- Item 1 of 1
Recombinant mouse IL-10 protein, N-His (HEK293) [orb1516603]
> 90% as determined by SDS-PAGE
20.6 kDa
500 μg, 100 μg 
IL-20 Protein, Mouse, Recombinant (His) [orb1959737]
SDS-PAGE: > 95%; SEC-HPLC: > 95%
19.5 kDa (predicted). Due to glycosylation, the protein migrates to 20-22 kDa based on Tris-Bis PAGE result.
1 mg, 100 μg, 500 μg
