Mouse IgG2a Isotype Control Antibody

SKU: orb1822664

Description

To serve as a true negative control for various procedures, this rabbit monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.

Images & Validation

• Tested Applications: ELISA, FACS, IF, IHC-P, WB
• Dilution Range: ELISA: assay dependent,Flow Cytometry: assay dependent,Immunofluorescence: assay dependent,Western blot: assay dependent,Immunohistochemistry (FFPE): assay dependent
• Application Notes:
  Optimal dilution of the Mouse IgG2a Isotype Control antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: IGG2a/6723
• Immunogen: Recombinant full-length human IG1R protein was used as the immunogen for the Mouse IgG2a Isotype Control antibody.
• Purification: Protein A affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human tonsil tissue with Mouse IgG2a Isotype Control antibody (clone IGG2a/6723). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Mouse IgG2a Isotype Control antibody (IGG2a/6723) as confirmation of integrity and purity.