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Catalog Number | orb1822664 |
---|---|
Category | Antibodies |
Description | To serve as a true negative control for various procedures, this rabbit monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | IGG2a/6723 |
Tested applications | ELISA, FACS, IF, IHC-P, WB |
Isotype | Mouse IgG2a |
Immunogen | Recombinant full-length human IG1R protein was used as the immunogen for the Mouse IgG2a Isotype Control antibody. |
Antibody Type | Primary Antibody |
Dilution range | ELISA: assay dependent,Flow Cytometry: assay dependent,Immunofluorescence: assay dependent,Western blot: assay dependent,Immunohistochemistry (FFPE): assay dependent |
Conjugation | Unconjugated |
Formula | 1 mg/ml in 1X PBS; BSA free, sodium azide free |
Hazard Information | This Mouse IgG2a Isotype Control antibody is available for research use only. |
Storage | Aliquot the Mouse IgG2a Isotype Control antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human tonsil tissue with Mouse IgG2a Isotype Control antibody (clone IGG2a/6723). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Mouse IgG2a Isotype Control antibody (IGG2a/6723) as confirmation of integrity and purity.
In vivo | |
Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
In vivo | |
Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, SDS-PAGE | |
Unconjugated | |
Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Confirmed by SDS-PAGE. | |
Mouse |
ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB | |
Monoclonal | |
Biotin |