Mouse IgG2a Fc Protein

• Catalog Number: orb257575
• Category: Proteins
• Description: Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.
• Reactivity: Mouse
• Tag: Native
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 26.4 kDa
• Target: IgG2a Fc
• Protein Sequence: CAC20702
• Source: Mouse IgG2a Fc, Tag Free (orb257575) is expressed from human 293 cells (HEK293). It contains AA Glu 98 - Lys 330 (Accession # CAC20702).
• Expression System: HEK293
• Expression Region: Glu 98 - Lys 330
• Endotoxins: 1.0 EU per μg
• NCBI: CAC20702
• Storage: -20℃
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5
• Alternative names: IgG2A
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 26.4 kDa. As a result of glycosylation, the protein migrates as 30-35 kDa under reducing (R) condition, and 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Mouse IgG Fc protein