Mouse IgG1 isotype Control Antibody

SKU: orb343776

Description

Mouse IgG1 isotype Control Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: ELISA, FC, SDS-PAGE
• Dilution Range: FC: 1:1000-1:5000
• Application Notes:
  Mouse IgG1 isotype control has been tested by ELISA and SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Key Properties

• Isotype: IgG1
• Clone No.: MG1
• Purity: Mouse IgG1 Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG1 and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mouse IgG1 isotype, Mouse IgG1 subclass isotype, Mouse IgG1 Control, Mouse isotype Control

SDS-PAGE of Mouse IgG1 Isotype Control. Lane 1: Mouse IgG1 Isotype Control, Non-reduced. M: Opal Pre-stained Ladder. Lane 2: Mouse IgG1 Isotype Control, Reduced. Load: 1.0 µg per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.