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Mouse IgG1 Fc Protein
Catalog Number: orb257565
| Catalog Number | orb257565 |
|---|---|
| Category | Proteins |
| Description | Mouse IgG Fc, Tag Free is expressed from human 293 cells (HEK293). It contains AA Val 111 - Lys 324 (Accession # AAK53870.1). |
| Target | IgG1 Fc |
| Conjugation | Unconjugated |
| Tag | Native |
| Reactivity | Mouse |
| Form/Appearance | Powder |
| Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5 |
| Purity | 95% |
| Protein Sequence | AAK53870.1 |
| MW | 24.3 kDa |
| Application notes | This protein carries no "tag". The protein has a calculated MW of 24.3 kDa. The protein migrates as 28-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Mouse |
| Expression Region | Val 111 - Lys 324 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | AAK53870.1 |
SDS-PAGE analysis of Mouse IgG Fc protein
Mouse IgG Fc protein
Mouse IgG Fc protein
- Item 1 of 2
- Item 1 of 1
Recombinant mouse IgG1 Fc protein, (HEK293) [orb1975970]
> 90% as determined by SDS-PAGE
25.7 kDa
1 mg, 100 μg - Item 1 of 1
Recombinant mouse IgG1 Fc protein (Isotype Control) (HEK293) [orb1516543]
> 95% as determined by SDS-PAGE
32 kDa
100 μgRecombinant mouse IgG1 Fc protein (HEK293) [orb1516388]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 34-36 kDa based on Tris-Bis PAGE result.
100 μg
